Makkuni Jayaram

The question we address is: what strategies does a parasite genome employ to ensure its stable propagation without jeopardizing the welfare of its host.

The model system we employ is the 2 micron plasmid of Saccharomyces cerevisiae. The plasmid has a copy number of approximately 60 per cell, and is rarely lost during cell division. The high plasmid stability is ensured by a partitioning system that includes two plasmid proteins and a cis-acting DNA locus. The plasmid also utilizes an amplification mechanism to compensate for rare stochastic downward fluctuations in copy number. A site-specific DNA recombination system encoded by the plasmid is responsible for this copy number control.

The research projects in the laboratory explore recombination mechanisms, test models for recombination mediated DNA amplification and examine plasmid segregation using tools of molecular genetics and cell biology.