Workshop: INTRO TO qPCR
Reverse Transcriptase quantitative PCR is a necessary tool for the discovery of genetic and expression level data or the validation of preliminary expression data derived from microarray experiments.
This workshop is designed to be an introduction to the first time qPCR user. The focus will be on the process of carrying out an experiment with some background of how quantitative PCR works. Fast SYBR® Green intercalating-dye chemistry from Applied Biosystems will be used to quantify relative expression levels of human genes in a cancerous disease state relative to normal tissue. Applied Biosystems (ABI) machines (the new ViiA 7 ®) will be used to collect data and anaylze results .
There are two options:
Note: if you are interested in future dates for the course, please email the instructor to put your email in the update list.
Class Size: ~ 6 participants
Location: Welch Hall biotech lab (WEL 3.270) and UT DNA Core Facility labs (MBB)
Pre-requisites for this course: basic understanding of standard PCR, pipetting skills
Registration & Further Information:
Instructor: Josh Beckham, Ph.D.
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To Register and make Payments, proceed to: TxShop
Payments can be made with Credit Card or UT Accounts or Grant Accounts.
Academic price requires proof of student status (i.e. current univeristy or school I.D. card)
If a class is full, you will receive an email about alternate dates.
Parking: the closest garage is the San Antonio Garage (SAG) - this is a paid garage (even on Saturdays)