Workshop: INTRO TO qPCR

Reverse Transcriptase quantitative PCR is a necessary tool for the discovery of genetic and expression level data or the validation of preliminary expression data derived from microarray experiments.

This workshop is designed to be an introduction to the first time qPCR user. The focus will be on the process of carrying out an experiment with some background of how quantitative PCR works. SYBR® Green intercalating-dye chemistry will be used to quantify relative expression levels of human genes in a cancerous disease state relative to normal tissue. Applied Biosystems (ABI) machines (the new ViiA 7 ®) will be used to collect data.

There are two options:

Class Size: ~ 6 participants

Location: Welch Hall biotech lab (WEL 3.270) and UT DNA Core Facility labs (MBB)

Pre-requisites for this course: basic understanding of standard PCR, pipetting skills

Registration & Further Information:

Instructor: Josh Beckham, Ph.D.

Tel: 471-5022

email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

Would you lik to receive emails about upcoming dates?

Sign up for the UTList:

https://utlists.utexas.edu/sympa/subscribe/rt-qpcr-workshop

Price:

To Register and make Payments, proceed to: TxShop

Payments can be made with Credit Card or UT Accounts or Grant Accounts.

Academic price requires proof of student status (i.e. current univeristy or school I.D. card)

If a class is full, you will receive an email about alternate dates.

Parking: the closest garage is the San Antonio Garage (SAG) - this is a paid garage (even on Saturdays)